Few other areas of society are currently undergoing as many changes as the mobility of people and goods. A number of (global) large-scale social trends, in very different ways, affect established social practices of moving around, transporting things, and connecting or demarcating places and territories. The digitalisation of transport, the restricted freedom of movement due to the pandemic, border closures and trade conflicts, alongside migration, climate change and civic engagement, increasingly overlap and change the shape and use of infrastructures and spatial orders that have evolved over decades. Certainly, this process can lead to tensions and conflict, as those firmly established are often pitted against outsiders, the privileged against the discriminated, or traditionalists against innovators. Societal debates about expanding bike lanes, a move away from the combustion engine, or tax increases on air travel make it clear that different cultural and economic milieus are currently renegotiating spatial cultures and mobility practices. In our blog series “Mobility and Conflict”, we will present contributions from different disciplinary perspectives that explore how complex, conflictual and ambivalent these processes of change can be, and how they may lead to new spatial cultures and mobility practices.